abstract：:Poly(ADP-ribose) polymerase-1 (PARP1) is a nuclear enzyme that can trigger caspase-independent necrosis. Two main mechanisms for this have been proposed: one involving RIP1 and JNK kinases and mitochondrial permeability transition (MPT), the other involving calpain-mediated activation of Bax and mitochondrial release ...

journal_title：Journal of cell science

authors： Douglas DL,Baines CP

更新日期：2014-10-01 00:00:00

abstract：:Iron (Fe(2+), Fe(3+)) homeostasis is a tightly regulated process, involving precise control of iron influx and egress from cells. Although the mechanisms of its import into cells by iron carrier molecules are well characterized, iron export remains poorly understood. The current paradigm envisages unique functions ass...

journal_title：Journal of cell science

authors： Sheokand N,Malhotra H,Kumar S,Tillu VA,Chauhan AS,Raje CI,Raje M

更新日期：2014-10-01 00:00:00

abstract：:Cutaneous wound healing is accelerated by exogenous mechanical forces and is impaired in TRPC6-knockout mice. Therefore, we designed experiments to determine how mechanical force and TRPC6 channels contribute to wound healing using HaCaT keratinocytes. HaCaT cells were pretreated with hyperforin, a major component of ...

journal_title：Journal of cell science

authors： Takada H,Furuya K,Sokabe M

更新日期：2014-10-01 00:00:00

abstract：:Chloride intracellular channel 3 (CLIC3) drives invasiveness of pancreatic and ovarian cancer by acting in concert with Rab25 to regulate the recycling of α5β1 integrin from late endosomes to the plasma membrane. Here, we show that in two estrogen receptor (ER)-negative breast cancer cell lines, CLIC3 has little influ...

journal_title：Journal of cell science

authors： Macpherson IR,Rainero E,Mitchell LE,van den Berghe PV,Speirs C,Dozynkiewicz MA,Chaudhary S,Kalna G,Edwards J,Timpson P,Norman JC

更新日期：2014-09-15 00:00:00

abstract：:The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here, we show that staurosporine causes defined changes in cytosolic free Ca(2+) ([Ca(2+)]c) dynamics and a distinct Ca(2+) signature that involves Ca(2+) influx from the external medium and internal Ca(2+) stores. We i...

journal_title：Journal of cell science

authors： Gonçalves AP,Cordeiro JM,Monteiro J,Muñoz A,Correia-de-Sá P,Read ND,Videira A

更新日期：2014-09-01 00:00:00

abstract：:Skeletal and heart muscle-specific variant of the α subunit of nascent polypeptide associated complex (skNAC; encoded by NACA) is exclusively found in striated muscle cells. Its function, however, is largely unknown. Previous reports have demonstrated that skNAC binds to m-Bop/Smyd1, a multi-functional protein that re...

journal_title：Journal of cell science

authors： Berkholz J,Michalick L,Munz B

更新日期：2014-09-01 00:00:00

abstract：:In many physiological contexts, intracellular reduction-oxidation (redox) conditions and the unfolded protein response (UPR) are important for the control of cell life and death decisions. UPR is triggered by the disruption of endoplasmic reticulum (ER) homeostasis, also known as ER stress. Depending on the duration a...

journal_title：Journal of cell science

authors： Eletto D,Chevet E,Argon Y,Appenzeller-Herzog C

更新日期：2014-09-01 00:00:00

abstract：:Defects in the biogenesis of the spindle pole body (SPB), the yeast centrosome equivalent, can lead to monopolar spindles and mitotic catastrophe. The KASH domain protein Kms2 and the SUN domain protein Sad1 colocalize within the nuclear envelope at the site of SPB attachment during interphase and at the spindle poles...

journal_title：Journal of cell science

authors： Wälde S,King MC

更新日期：2014-08-15 00:00:00

abstract：:Loss-of-function mutations in the genes encoding PINK1 and Parkin (also known as PARK2) are the most common causes of recessive Parkinson's disease. Both together mediate the selective degradation of mitochondrial proteins and whole organelles via the proteasome and the autophagy-lysosome pathway (mitophagy). The mito...

journal_title：Journal of cell science

authors： Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

更新日期：2014-08-15 00:00:00

abstract：:Regulation of the localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae, localization of mRNAs that encode membrane proteins requires the She protein machinery, including the RNA-binding protein She2p, as w...

journal_title：Journal of cell science

authors： Hermesh O,Genz C,Yofe I,Sinzel M,Rapaport D,Schuldiner M,Jansen RP

更新日期：2014-08-01 00:00:00

abstract：:Here, we identify a role for the matrilin-2 (Matn2) extracellular matrix protein in controlling the early stages of myogenic differentiation. We observed Matn2 deposition around proliferating, differentiating and fusing myoblasts in culture and during muscle regeneration in vivo. Silencing of Matn2 delayed the express...

journal_title：Journal of cell science

authors： Deák F,Mátés L,Korpos E,Zvara A,Szénási T,Kiricsi M,Mendler L,Keller-Pintér A,Ozsvári B,Juhász H,Sorokin L,Dux L,Mermod N,Puskás LG,Kiss I

更新日期：2014-08-01 00:00:00

abstract：:Dynamic modulation of the physical contacts between neighboring cells is integral to epithelial processes such as tissue repair and cancer dissemination. Induction of matrix metalloproteinase (MMP) activity contributes to the disassembly of intercellular junctions and the degradation of the extracellular matrix, thus ...

journal_title：Journal of cell science

authors： Mauris J,Woodward AM,Cao Z,Panjwani N,Argüeso P

更新日期：2014-07-15 00:00:00

abstract：:By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell-cell contact and transcriptional regulation of proliferation, coordinating epithelial tissue homeostasis and regeneration. The integration of multiple growth-regulatory inputs with ...

journal_title：Journal of cell science

authors： Georgopoulos NT,Kirkwood LA,Southgate J

更新日期：2014-07-01 00:00:00

abstract：:BAG6 participates in protein quality control and, here, we address its role in endoplasmic-reticulum-associated degradation (ERAD) by using the polytopic membrane protein OpD, an opsin degron mutant. Both BAG6 knockdown and BAG6 overexpression delay OpD degradation; however, our data suggest that these two perturbatio...

journal_title：Journal of cell science

authors： Payapilly A,High S

更新日期：2014-07-01 00:00:00

abstract：:Interferon regulatory factor 6 (Irf6) regulates keratinocyte proliferation and differentiation. In this study, we tested the hypothesis that Irf6 regulates cellular migration and adhesion. Irf6-deficient embryos at 10.5 days post-conception failed to close their wound compared with wild-type embryos. In vitro, Irf6-de...

journal_title：Journal of cell science

authors： Biggs LC,Naridze RL,DeMali KA,Lusche DF,Kuhl S,Soll DR,Schutte BC,Dunnwald M

更新日期：2014-07-01 00:00:00

abstract：:Crosstalk between the microtubule (MT) and actin cytoskeletons is fundamental to many cellular processes including cell polarisation and cell motility. Previous work has shown that members of the growth-arrest-specific 2 (GAS2) family mediate the crosstalk between filamentous actin (F-actin) and MTs, but the molecular...

journal_title：Journal of cell science

authors： Stroud MJ,Nazgiewicz A,McKenzie EA,Wang Y,Kammerer RA,Ballestrem C

更新日期：2014-06-15 00:00:00

abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

journal_title：Journal of cell science

authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

更新日期：2014-06-01 00:00:00

abstract：:NEDD8 is an important regulatory factor in many biological processes. However, the substrates for neddylation, and the relationship between the ubiquitin and NEDD8 pathways remain largely unknown. Here, we show that NEDD8 is covalently conjugated to histone 2A (H2A), and that neddylation of H2A antagonizes its ubiquit...

journal_title：Journal of cell science

authors： Li T,Guan J,Huang Z,Hu X,Zheng X

更新日期：2014-05-15 00:00:00

abstract：:Regulated, programmed cell death is crucial for all multicellular organisms. Cell death is essential in many processes, including tissue sculpting during embryogenesis, development of the immune system and destruction of damaged cells. The best-studied form of programmed cell death is apoptosis, a process that require...

journal_title：Journal of cell science

authors： Tait SW,Ichim G,Green DR

更新日期：2014-05-15 00:00:00

abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...

journal_title：Journal of cell science

authors： Lowndes M,Rakshit S,Shafraz O,Borghi N,Harmon RM,Green KJ,Sivasankar S,Nelson WJ

更新日期：2014-05-15 00:00:00

abstract：:Retinoic acid (RA) plays key roles in cell differentiation and growth arrest by activating nuclear RA receptors (RARs) (α, β and γ), which are ligand-dependent transcription factors. RARs are also phosphorylated in response to RA. Here, we investigated the in vivo relevance of the phosphorylation of RARs during RA-ind...

journal_title：Journal of cell science

authors： Al Tanoury Z,Gaouar S,Piskunov A,Ye T,Urban S,Jost B,Keime C,Davidson I,Dierich A,Rochette-Egly C

更新日期：2014-05-01 00:00:00

abstract：:Proper cellular functioning requires that cellular machinery behave in a spatiotemporally regulated manner in response to global changes in nutrient availability. Mounting evidence suggests that one way this is achieved is through the establishment of physically defined gradients of O-GlcNAcylation (O-linked addition ...

journal_title：Journal of cell science

authors： Harwood KR,Hanover JA

更新日期：2014-05-01 00:00:00

abstract：:Connexin 30 (Cx30), a member of the large gap-junction protein family, plays a role in the homeostasis of the epidermis and inner ear through gap junctional intercellular communication (GJIC). Here, we investigate the underlying mechanisms of four autosomal dominant Cx30 gene mutations that are linked to hearing loss ...

journal_title：Journal of cell science

authors： Berger AC,Kelly JJ,Lajoie P,Shao Q,Laird DW

更新日期：2014-04-15 00:00:00

abstract：:Embryonal rhabdomyosarcomas (ERMSs) show elevated levels of PAX7, a transcription factor that marks quiescent adult muscle stem (satellite) cells and is important for proliferation and survival of activated satellite cells and whose timely repression is required for myogenic differentiation. However, the mechanism of ...

journal_title：Journal of cell science

authors： Riuzzi F,Sorci G,Sagheddu R,Sidoni A,Alaggio R,Ninfo V,Donato R

更新日期：2014-04-15 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:Cdc6 and Cdt1 initiate DNA replication licensing when cells exit mitosis. In cycling cells, Cdc6 is efficiently degraded from anaphase onwards as a result of APC/C-Cdh1 activity. When APC/C-Cdh1 is switched off again, at the end of G1 phase, Cdc6 could thus re-accumulate, risking the re-licensing of DNA as long as Cdt...

journal_title：Journal of cell science

authors： Clijsters L,Wolthuis R

更新日期：2014-03-15 00:00:00

abstract：:TAR DNA-binding protein (TDP-43, also known as TARDBP) is the major pathological protein in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Large TDP-43 aggregates that are decorated with degradation adaptor proteins are seen in the cytoplasm of remaining neurons in ALS and FTD patients post mor...

journal_title：Journal of cell science

authors： Scotter EL,Vance C,Nishimura AL,Lee YB,Chen HJ,Urwin H,Sardone V,Mitchell JC,Rogelj B,Rubinsztein DC,Shaw CE

更新日期：2014-03-15 00:00:00

abstract：:Electrical synapses (gap junctions) rapidly transmit signals between neurons and are composed of connexins. In neurons, connexin36 (Cx36) is the most abundant isoform; however, the mechanisms underlying formation of Cx36-containing electrical synapses are unknown. We focus on homocellular and heterocellular gap juncti...

journal_title：Journal of cell science

authors： Meyer A,Hilgen G,Dorgau B,Sammler EM,Weiler R,Monyer H,Dedek K,Hormuzdi SG

更新日期：2014-03-15 00:00:00

abstract：:Phosphoinositides regulate myriad cellular processes, acting as potent signaling molecules in conserved signaling pathways and as organelle gatekeepers that recruit effector proteins to membranes. Phosphoinositide-generating enzymes have been studied extensively in yeast and cultured cells, yet their roles in animal d...

journal_title：Journal of cell science

authors： Tan J,Oh K,Burgess J,Hipfner DR,Brill JA

更新日期：2014-03-01 00:00:00

abstract：:The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC t...

journal_title：Journal of cell science

authors： Ichise T,Yoshida N,Ichise H

更新日期：2014-02-15 00:00:00

abstract：:Although many pathogenic copy number variations (CNVs) are associated with neuropsychiatric diseases, few of them have been functionally characterised. Here we report multiple schizophrenia cases with CNV abnormalities specific to unc-51-like kinase 4 (ULK4), a serine/threonine kinase gene. Deletions spanning exons 21...

journal_title：Journal of cell science

authors： Lang B,Pu J,Hunter I,Liu M,Martin-Granados C,Reilly TJ,Gao GD,Guan ZL,Li WD,Shi YY,He G,He L,Stefánsson H,St Clair D,Blackwood DH,McCaig CD,Shen S

更新日期：2014-02-01 00:00:00

abstract：:ADP-ribosylation factors (Arfs) play central roles in the regulation of vesicular trafficking through the Golgi. Arfs are activated at the Golgi membrane by guanine-nucleotide-exchange factors (GEFs) that are recruited from cytosol. Here, we describe a novel mechanism for the regulation of recruitment and activity of ...

journal_title：Journal of cell science

authors： Quilty D,Gray F,Summerfeldt N,Cassel D,Melançon P

更新日期：2014-01-15 00:00:00

abstract：:Members of the ezrin-radixin-moesin (ERM) family of proteins are involved in multiple aspects of cell migration by acting both as crosslinkers between the membrane, receptors and the actin cytoskeleton, and as regulators of signalling molecules that are implicated in cell adhesion, cell polarity and migration. Increas...

journal_title：Journal of cell science

authors： Clucas J,Valderrama F

更新日期：2014-01-15 00:00:00

abstract：:Cells synthesize ceramides in the endoplasmic reticulum (ER) as precursors for sphingolipids to form an impermeable plasma membrane. As ceramides are engaged in apoptotic pathways, cells would need to monitor their levels closely to avoid killing themselves during sphingolipid biosynthesis. How this is accomplished re...

journal_title：Journal of cell science

authors： Tafesse FG,Vacaru AM,Bosma EF,Hermansson M,Jain A,Hilderink A,Somerharju P,Holthuis JC

更新日期：2014-01-15 00:00:00

abstract：:Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski is a negative regulator of TGF-β-Smad signaling in myofibroblasts, and might redirect the myofibroblast phenotype back to fibroblasts. Meox2 could alter TGF-β-mediat...

journal_title：Journal of cell science

authors： Cunnington RH,Northcott JM,Ghavami S,Filomeno KL,Jahan F,Kavosh MS,Davies JJ,Wigle JT,Dixon IM

更新日期：2014-01-01 00:00:00

abstract：:Hematopoietic and vascular endothelial cells constitute the circulatory system and are both generated from the ventral mesoderm. However, the molecules and signaling pathways involved in ventral mesoderm formation and specification remain unclear. We found that zebrafish etv5a was expressed in the ventral mesoderm dur...

journal_title：Journal of cell science

authors： Chen SY,Shih HY,Lin SJ,Hsiao CD,Li ZC,Cheng YC

更新日期：2013-12-15 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:Non-muscle myosin II (MyoII) contractility is central to the regulation of numerous cellular processes, including migration. Rho is a well-characterized modulator of actomyosin contractility, but the function of other GTPases, such as Rac, in regulating contractility is currently not well understood. Here, we show tha...

journal_title：Journal of cell science

authors： Jean L,Majumdar D,Shi M,Hinkle LE,Diggins NL,Ao M,Broussard JA,Evans JC,Choma DP,Webb DJ

更新日期：2013-12-15 00:00:00

abstract：:A number of recent studies have highlighted the importance of autophagy and the ubiquitin-proteasome in the pathogenesis of muscle wasting in different types of inherited muscle disorders. Autophagy is crucial for the removal of dysfunctional organelles and protein aggregates, whereas the ubiquitin-proteasome is impor...

journal_title：Journal of cell science

authors： Sandri M,Coletto L,Grumati P,Bonaldo P

更新日期：2013-12-01 00:00:00

abstract：:Quantitative information about the range of influence of extracellular signaling molecules is critical for understanding their effects, but is difficult to determine in the complex and dynamic three-dimensional environment of a living embryo. Drosophila germ cells migrate during embryogenesis and use spatial informati...

journal_title：Journal of cell science

authors： Mukherjee A,Neher RA,Renault AD

更新日期：2013-12-01 00:00:00